From e72b8b4af6df0023b7aec9e5ec00837f9d288144 Mon Sep 17 00:00:00 2001
From: Ayushi Agrawal
 <88406934+ayushi-agrawal-gladstone@users.noreply.github.com>
Date: Wed, 9 Mar 2022 14:45:11 -0800
Subject: [PATCH] Delete all_steps.sh

---
 intro-rna-seq/all_steps.sh | 28 ----------------------------
 1 file changed, 28 deletions(-)
 delete mode 100644 intro-rna-seq/all_steps.sh

diff --git a/intro-rna-seq/all_steps.sh b/intro-rna-seq/all_steps.sh
deleted file mode 100644
index d404af0..0000000
--- a/intro-rna-seq/all_steps.sh
+++ /dev/null
@@ -1,28 +0,0 @@
-#!/bin/bash
-
-fastqc Bacteria_GATTACA_L001_R1_001.fastq
-
-cutadapt \
--a file:Adapter_Sequence.fasta \
--o trimmed.fastq \
-Bacteria_GATTACA_L001_R1_001.fastq
-
-fastqc trimmed.fastq
-
-mkdir star_index
-
-STAR \
---runMode genomeGenerate \
---genomeDir ./star_index \
---genomeFastaFiles rDNA_sequence.fasta \
---genomeSAindexNbases 3
-
-STAR \
---genomeDir ./star_index \
---readFilesIn ./trimmed.fastq
-
-featureCounts \
--a rDNA.gtf \
--t CDS \
--o counts.txt \
-Aligned.out.sam